FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345673

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345673
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2313428
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9100.038.7675138
TCGCTCT10400.032.537024
GTCTCGC9850.031.5186391
GTCTTGC19850.030.9167391
GTATAGA7150.028.2739961
CTGTCGC13150.027.9227189
CGCTCTG12100.027.3706915
GTATAAA9650.026.9345931
CTCGCTC12600.026.318113
TAATACT7950.026.2629874
GTCTCAC28350.025.97661
ACCGTGT8050.025.936748
GTGTTAG17900.025.8144071
ATGGCGT10000.025.1988836
GTATTAA7450.025.1972031
GTGTTAC10700.024.9662421
GGATCGT2901.2840246E-824.8264878
GTGTAAC8450.024.7785871
GTATAAG7300.024.7259161
CCGTGTT8750.024.6846229
Produced by FastQC (version 0.11.5)