FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345674

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345674
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2313428
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC10950.048.000851
TCTGTCG11400.044.2103548
TCGCTCT12100.040.4626544
CGCTCTG12900.037.953345
CTGTCGC14400.035.499869
TCTCGCT14050.035.3600542
CTCGCTC13750.034.0362323
GTCTTAC10150.032.631121
GTGTTAC9400.032.170781
GTCTTGC22150.031.8560941
GGGTTTC39650.030.6890681
GGGTACG2601.200533E-1030.462081
GTTTCAC35450.029.2467143
GGCGTAC1255.3684495E-428.800511
GTACGAC1255.3684495E-428.800511
TATACGG1000.006285127728.7998875
GTATAAC4500.025.6004541
GGTTTCA38600.025.554862
GTGTAAC7750.024.1552661
TTGCTCT30100.023.9201724
Produced by FastQC (version 0.11.5)