FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345676

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345676
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2321228
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC10150.036.886711
TCTGTCG11000.036.6537638
GTCTTGC21600.034.666681
TCGCTCT12900.032.3714034
GTCCGTA1153.2948022E-431.3036829
GTATAAC3002.0008883E-1128.8000091
CTGTCGC14750.028.7993899
TAATACT6550.028.5795464
GTATTAG7750.027.8709751
GTATTAC5700.027.7894821
CTCGCTC14350.027.5952323
GTGTAAA15700.027.5159321
GTGTAAC7100.027.3802911
TCTCGCT14900.026.577192
TTGCTCT28550.025.974934
GTACTAT5950.025.4117721
GTCTCAC25450.024.8958821
ATACCGT1751.3170521E-424.685196
CTTGCTC29300.024.5728573
CGCTCTG14250.024.2521155
Produced by FastQC (version 0.11.5)