FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345677

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345677
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2042256
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8400.053.9982768
GTCTCGC8750.042.894321
GTCTTGC19650.041.506911
CTGTCGC11900.039.9314969
TCGCTCT10000.037.4388054
TCTCGCT10750.034.187182
TTGCTCT26150.033.3144234
CGCTCTG11750.030.0245725
CTCGCTC12500.029.9774653
GTATAAC2901.2732926E-1129.8667341
GTCTCAC23800.029.720331
CTTGCTC26450.028.3340873
GTATTAG6650.028.219951
GGTATAC4100.028.1670041
GTCCTAC5500.026.2465231
CTGTTGC21700.026.2111439
TAGCGGT1658.830603E-526.1809834
GGGTTTC29950.026.027441
GTGTTAG14700.026.023341
GTTTCAC27500.025.9420363
Produced by FastQC (version 0.11.5)