FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345678

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345678
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2042256
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC9250.049.8174171
TCTGTCG11200.048.857138
GTCTTGC19250.043.388061
CTGTCGC13600.042.882349
TCGCTCT10000.039.5999874
ATACCGT1201.0033633E-535.999996
GGTATAC4350.034.759461
CTCGCTC11200.034.7142753
TCTCGCT11800.032.3397642
CGCTCTG11700.031.3846045
GTGTTAG13750.030.895291
TCTGTCA23950.029.4613698
TTGCTCT24800.029.322574
TACGTAC1000.00628484928.799993
GTATTGC5200.027.6929781
GTCCTAT5250.027.4292341
CTTGCTC25050.026.730533
GTTAGCC11350.026.6431623
GTATAAC3555.456968E-1226.3668351
GTCTCAC24200.025.8849221
Produced by FastQC (version 0.11.5)