FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345679

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345679
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2347052
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTCT10500.032.2267844
GTCTTGC20650.032.1682171
TCTGTCG9700.031.9171148
GTCTCGC10500.030.9444261
GTGTTAC8400.030.9444241
GTCTCAC24950.030.0969161
GCGTAGT2651.4551915E-1029.9713331
GTACTAC4150.029.5774021
GTGTAAA12700.029.5637041
TCTCGCT11850.026.7663362
GTGTAAC7100.026.4407771
GTATTAG7200.026.0735441
GTATAGC5350.025.642421
GTGTTAG16850.024.8534851
CTGTCGC12850.024.653389
GTATAAG6750.024.602731
GTCTTAC8900.024.3383121
GTTAGCC13500.024.0288723
CGTAGTG3601.9826984E-1024.028872
GTGTAGG15500.023.7573321
Produced by FastQC (version 0.11.5)