FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345680

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345680
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2347052
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10300.039.844288
GTCTCGC10500.038.401271
GTCTTGC21300.035.4941331
GTATCGG1301.7356288E-533.2318731
TCGCTCT12000.032.999694
TCTCGCT13250.031.5180262
TATCGGC1153.2937626E-431.3053872
CTGTCGC13200.031.0906169
CGCTCTG12650.030.7348865
CTCGCTC13900.028.488943
CTACGCA2054.2956708E-728.0972949
GTCTTAC8600.027.6288221
GGTATAC3700.027.2441461
CTCGATC9550.024.8793458
TTGCTCT30150.024.3579794
GTACTAG3602.0190782E-1024.0007951
GGCTAGA13350.023.7311211
GTATAAC4303.6379788E-1223.4426381
GGGTTTC36250.023.4380171
GTTTCAC32200.023.254443
Produced by FastQC (version 0.11.5)