FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345681

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345681
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1898596
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG7400.044.7553638
GTCTCGC9650.040.3739621
TCGCTCT9550.039.202974
CCGATCG750.001535278538.3988089
GTCTTGC18400.037.2512441
TCTCGCT10150.036.2057722
CTCGCTC11350.032.377853
CTGTCGC11850.032.201539
CGCTCTG11050.031.9266075
ACTGTCG2853.5106495E-1027.7886098
TTGCTCT24950.026.8368424
TACTCCG1907.5570733E-626.5254925
GTATTAC5500.024.9244671
TCCGTAC1751.3097345E-424.7051163
GTGTAAA10250.024.6365091
GTATAGA4101.8189894E-1224.6365091
GTATAAC4101.8189894E-1224.6365091
GTCTTAC6750.024.5843681
GTGTTAC7200.024.0499231
GTATAAT5100.024.0499231
Produced by FastQC (version 0.11.5)