FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345682

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345682
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1898596
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC9450.049.5236781
TCGCTCT10450.042.0286034
TCTGTCG9850.041.6648678
TCTCGCT11850.038.8859752
CTGTCGC12500.038.5919049
CGCTCTG11300.036.9556585
CTCGCTC11700.036.30763
GTCTTGC16700.034.9220661
GTATTAG5700.032.8420221
GCGTTAG1851.7479033E-731.1350561
GTATTAC4750.028.7999251
GTGTTAG12150.028.4443721
TTACACT8400.028.2856434
TTGCTCT23800.026.016744
GTATAAC3607.2759576E-1225.9999311
TCTGTCA24450.025.325098
GGGTTTC32500.025.2553181
CTGTTGC20700.025.0434139
GTTTCAC29050.024.7847883
ACCATGT25450.024.3299968
Produced by FastQC (version 0.11.5)