FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345683

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345683
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2369393
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC10100.043.5852431
GTCTTGC20650.039.1407361
TCTGTCG9400.038.2985348
TCGCTCT10950.036.8202254
CGTTTCG1406.981991E-736.000629
CTGTCGC12250.035.853689
TCTCGCT12850.031.4097922
GGGTTTC38600.030.4741291
GTATTAG7650.030.186971
CGCTCTG12300.029.8522855
CTCGCTC12800.028.1540073
GTTTCAC36800.028.0071143
GTGTTAC9950.027.560781
GTGTTAG15600.027.2934471
GTATAAA9250.026.525781
GTATAAC4650.026.383171
GGTTTCA37800.025.9314792
TTGCTCT31950.025.4636174
TTCACCG6600.025.0897565
CTGTTGC25300.025.0439119
Produced by FastQC (version 0.11.5)