FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345687

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345687
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2985233
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11650.042.643318
GTCTCGC13700.039.9726331
GTCTTGC31500.034.769851
CTGTCGC14900.032.3754849
TCGCTCT15700.032.1026344
TCTCGCT15600.031.3853262
CTCGCTC16450.028.4504873
GTATTAC8000.027.9216751
GTATAAC6300.026.3061351
TTGCTCT42100.025.6537864
GTGTAAA19200.025.5198121
GTGTTAG19900.025.346311
CGCTCTG17750.025.149865
GTCTTAC12900.025.1357921
GTCTCAC36900.024.4091911
GTGTTAT13450.024.1079351
CTTGCTC40700.023.8826053
GTATATA13050.023.1904161
GTATTAT8400.023.1608371
TACACTG19000.023.116315
Produced by FastQC (version 0.11.5)