FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345688

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345688
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2985233
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12150.045.629448
CTGTCGC15400.037.4024439
GTCTCGC13100.037.383281
GTCTTGC29750.034.3748781
TCGCTCT14800.030.1630484
GTATTAT9900.027.6431921
TCTCGCT17550.027.4939732
GGTATAC6100.027.154251
CTCGCTC17000.026.6831363
GTCTTAC12800.026.4440351
GTGTTAG20900.025.4991251
CGCTCTG15050.025.3562265
TCTATCG2351.4147063E-624.5105348
GTGTTAC14100.024.0059321
GTCGTAC1500.001554200824.005931
GTGTAAC9100.023.742131
GTATAAA11850.023.7020571
GTCTCAC38250.023.3469431
GTACTAT6550.023.0896741
TTGCTCT39200.022.9598564
Produced by FastQC (version 0.11.5)