FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345689

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345689
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2884765
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11950.046.391898
CTGTCGC14300.040.7819759
GTCTTGC26850.039.7238121
GTCGTAC1553.662717E-837.1956181
GTCTCGC13300.035.7623251
TCGCTCT14600.033.040664
TTGCTCT37250.028.6063354
CTCGCTC16400.028.5362113
GTATAAC6400.028.1509821
TCTCGCT16750.027.5100882
GTATTAA9250.027.268411
GGTATAC6400.025.8989031
CTTGCTC38100.025.5114773
TCTTGCT42200.025.080242
GTGTTAG19200.024.7728611
CGCTCTG17750.024.7433385
GTGTAAC9700.024.5174731
GGGTTTC50600.023.7847961
GTCTCAC32000.023.4216141
GTACCGA1600.00226560522.4997066
Produced by FastQC (version 0.11.5)