FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345691

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345691
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2837853
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13000.043.7527668
GTCTCGC12000.037.822391
CTGTCGC15550.035.6518559
TCGCTCT13900.032.1143154
GTCTTGC27050.031.6934381
GTATTAG9350.030.8203831
TTCACCG8450.030.6737985
GTATAAT7200.030.0177691
TACCCGT1753.987725E-628.7992885
TCTCGCT16300.028.7109472
GTATTAA8700.028.1545961
GTCTTAC10550.027.3147491
CGCTCTG16050.026.9152225
GTTTCAC45150.026.6305713
CTCGCTC16950.025.9108643
GTGTTAG18450.025.3808771
GGGTTTC49600.024.982531
GTCTCAC34400.024.921731
GTCGTAA1500.001551062324.0142151
GTGTAAC9800.023.524131
Produced by FastQC (version 0.11.5)