FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345696

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345696
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2866385
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC13200.037.6441151
TCTGTCG12550.034.996518
TCGCTCT15050.032.532594
GTCTTGC26550.030.650381
CTGTCGC15300.030.1185979
TCTCGCT16000.027.9057452
GTATAGA10050.027.2294881
CTCGCTC18350.026.682043
GTGCGTA2508.368443E-825.9253391
GTATACG1959.230631E-625.8514791
GTACTAT6150.025.7614021
CGCTCTG17850.025.0091925
TAATACT8600.024.2798334
TTGCTCT37600.024.128424
GGTATAC5800.023.5910661
ATACCGT2502.4228138E-623.0403236
GCGTAGT3503.790774E-922.6332321
TACACCG2252.79881E-522.4007055
GCGCTAA1650.002705458821.8226761
GTATTAC7000.021.604451
Produced by FastQC (version 0.11.5)