FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345697

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345697
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2688598
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10250.045.6580778
GTCTCGC12050.040.060361
TCGCTCT13250.036.4078564
CTGTCGC13400.035.462339
TCTCGCT14550.032.1652222
GTCTTGC27750.031.6755311
CGCTCTG14200.031.4368885
CTCGCTC15400.030.8574053
GTGTAAC9250.026.482821
GTATTAT8850.026.0515631
GTTTCAC43850.024.7938273
GGGTTTC48300.024.1654531
GTGTTAG18250.024.0820831
GTCTTAC11100.024.0162831
GTCTCAC34900.023.5345821
TTGCTCT37000.023.1569544
GTATAGT8800.022.9246351
GGGTACG2903.4943332E-722.359991
CTTGCTC36950.022.2139913
GTATAAG8900.021.8575171
Produced by FastQC (version 0.11.5)