FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345698

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345698
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2688598
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTGC27000.033.8716051
TCGCTCT12100.030.3470174
TCTGTCG12650.030.1659188
GTATAGA8900.027.5096281
GTCTCGC11150.027.1250321
GTATTAT8800.027.0039391
GTATTAC5450.025.1045781
GTATTAG10100.024.9541341
GTGTTAG20200.023.884671
TAATACT9700.023.7525064
CTGTCGC15950.023.0218759
GTCTTAC11050.022.8087561
TTACACT15900.022.1886144
CGCTCTG14150.021.8797935
GTTTCAC45200.021.8229453
GTATAGC6300.021.7174511
GTGTAAC9450.021.3364431
GGGTTTC49350.021.15811
GTGTTAT11650.021.015941
GTGTTAC12000.021.0030611
Produced by FastQC (version 0.11.5)