FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345699

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345699
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2720178
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC12900.044.1251221
TCTGTCG10650.041.2391178
TCGCTCT13100.036.8241424
CTGTCGC13600.036.5291259
CGCTCTG14150.034.6004375
GTCTTGC24950.032.344171
TCTCGCT15300.031.9997482
CTCGCTC15800.029.164333
GTATTAG8500.026.2779391
GTATCCG1658.7861496E-526.200881
GTGTAGA14850.024.7452741
GTGTTAG18150.024.2159651
GTATTAC6950.023.8446851
GTTTCAC43100.023.7213943
GGGTTTC50300.023.2057481
GTCTCAC35550.023.1054171
GTGTAGG17300.022.9068371
GTATAGG8100.022.23841
ACCGTGT8150.022.0857168
GTATAAC5250.021.9588321
Produced by FastQC (version 0.11.5)