FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345703

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345703
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3115516
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13300.037.89458
GTCTCGC15150.037.578091
CTGTCGC16650.036.3240979
GTATTAG9850.033.65441
GTCTTGC29050.032.745231
ACTGTCG4050.031.99988
TCGCTCT16350.031.265864
GTATTAC6550.028.6056841
TCTCGCT20050.027.2916012
GTATTAA8900.026.7204781
CGCTCTG19250.026.1816545
GTGTATA15200.025.127691
GTGTAGG19350.024.9525011
GTCTCAC38150.024.9344441
CTCGCTC20300.024.827433
GTATAGA9150.023.6276451
GTATATA12300.022.8496631
TCACTCT42000.022.2855764
GTATTGG10100.022.118751
GTGTAAT13800.021.9326171
Produced by FastQC (version 0.11.5)