FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345704

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345704
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3115516
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13850.039.508938
GTCTTGC29750.035.3406071
GTCTCGC12900.034.052441
CTGTCGC17650.033.042419
TCGCTCT14650.030.9623834
GGTATAC6100.027.152271
GTGTTAG19450.025.9171091
GTATAAT9250.025.6909581
CGCTCTG18800.025.6595125
TCTCGCT17400.025.2457752
GTATTAG11150.025.1882421
GTCTTAC11900.024.8110431
GTGTAAG15700.024.7686441
CTCGCTC17650.024.4758633
GTACTAC6100.023.6106681
TTGCTCT40000.023.2199444
TATCCGG1600.002265505522.4999456
TAGTACT6450.022.3255274
TCTGTCA38600.022.1968388
GTGTTAC11700.022.1577031
Produced by FastQC (version 0.11.5)