FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345705

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345705
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2970516
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11950.043.9821788
GTCTCGC13850.037.9881671
CTGTCGC15800.037.366179
TCGCTCT15350.033.772864
GTCTTGC26250.029.9276391
CGCTCTG16100.028.6218595
TCTCGCT16950.028.0361212
CTCGCTC17300.027.0527233
GTCTCAC29650.026.49581
GTACTAC3555.456968E-1226.393091
GTGTAAC7600.024.6567041
GGTATAC4455.456968E-1222.674791
GTACTAG5500.022.2772451
TTGCTCT34750.022.1703574
GTCTTAC10200.021.904671
CGGACTA1700.003220436421.1770175
ACCGTGT9600.020.9994818
GTGTAGC9300.020.924551
TCACTCT35200.020.8641764
GTATTAG8800.020.4754091
Produced by FastQC (version 0.11.5)