FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345706

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345706
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2970516
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC12350.044.895591
TCTGTCG13150.042.1600468
TCGCTCT15350.037.99384
GTCTTGC24050.034.7314451
CGCTCTG15450.033.0876545
TCTCGCT16600.032.0998952
CTGTCGC17400.031.862879
CTCGCTC16850.030.3385313
GTCTCAC31650.026.6190241
TCTGTCA33250.025.3355488
GTCTTAC8950.024.9412751
TTGCTCT35000.024.8916364
GTTCTAC7000.022.6310461
CTTGCTC34550.022.50673
CTGTCAC39100.022.097749
TCACTCT35750.021.952634
GTGTTAG17000.021.6023621
GTGTTAC10450.020.6721151
CGTTTCG1750.00380958520.5719459
GGCTAGA16550.020.4493661
Produced by FastQC (version 0.11.5)