FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345707

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345707
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2833742
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10600.044.8241128
GCGTACG657.5983943E-444.3016859
CTGTCGC13700.037.308819
GTCTTGC29700.034.4139061
GTCTCGC13400.033.7791941
TCGCTCT13700.032.0563134
TTCACCG8500.027.102215
GTATAAT9250.026.8348731
TCTCGCT15950.026.6324392
GTATACG2207.0520946E-726.5478691
GTATTAG10300.026.2256851
CTCGCTC16100.025.9371193
GTATTAA8400.024.3355461
GTTTCAC46100.023.8951683
GGTATAC4950.023.5981041
GTGTTAC13000.023.586761
TTGCTCT37600.022.7857554
CGCTCTG17150.022.6674825
GTGTATA15250.022.5004061
GTATTAC6250.022.1940171
Produced by FastQC (version 0.11.5)