FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345710

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345710
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3299896
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC15450.037.8828241
TCTGTCG17000.037.269068
GTCTTGC29550.033.176311
TCGCTCT17450.028.8795854
GTATTAT12350.027.8427241
CTGTCGC22800.027.4717259
GTGTTAG22950.025.82161
GTATAAC6750.024.9291
CGCTCTG18500.024.9067085
TCTCGCT20400.024.7029972
CTCGCTC20400.024.349363
GTGTAAT16700.023.2188241
GTATTAC8700.022.70521
GGTATAC6550.022.33931
GTCTCAC36950.022.1760621
GTTAGCC18100.021.8752373
TTACACT18450.021.8514374
GTATTGA11450.021.7247221
GTCTTAC14500.021.191521
GCGTAGT3808.1618055E-921.1782441
Produced by FastQC (version 0.11.5)