FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345711

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345711
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3026799
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11600.042.200628
GTCTCGC13550.037.2082751
TCGCTCT14700.036.731654
CTGTCGC15250.033.5163239
CGCTCTG15750.031.995775
GTCTTGC28150.029.0716291
TCTCGCT16800.028.2838422
CTCGCTC16400.028.0957013
GTATTAA8800.024.909691
GTATAAT8000.023.7472361
GTGTATA12100.022.9471071
GTATAGG6700.022.9020421
GTCTCAC30900.022.7008671
GTCCTAC7850.022.3393921
GTATTAT8200.022.2769581
GTCTAAT8200.022.2769581
TAATACT9700.022.26624
GTCGTGA3004.2308602E-721.9205251
GTGTTAC10300.021.2820641
GTATTAG10300.020.5726621
Produced by FastQC (version 0.11.5)