FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345712

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345712
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3026799
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG14300.033.729938
GTCTTGC25450.030.2670251
GTCTCGC12250.029.9434721
TCGCTCT14500.024.3279084
GTCTCAC33900.023.1554011
CTGTCGC19050.023.0513949
GTGTAAA17600.022.5086441
GCGTATA2002.8666868E-422.0084531
GTATTAT9000.022.008451
TTGCTCT37950.021.8153784
GTATTAG9500.021.6223371
CTTGCTC35400.021.1491263
CTCGCTC14150.020.8588223
GGAGTGC63150.020.634275
TCTCGCT16650.020.3217142
GTGTTAG21050.020.2136211
TATACTG9300.020.126785
GTATTAC7700.020.0076831
GTATAGC5900.019.8946441
GTCTTAC12550.019.874831
Produced by FastQC (version 0.11.5)