FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345713

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345713
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3110324
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC14200.031.850541
TCTGTCG11950.031.3271758
GTCTTGC30500.030.13591
CTGTCGC15100.029.5597349
GTATTAG11100.027.601951
GTGTAGT17100.026.4489861
GTGTTAG21050.026.3375261
TCGCTCT15750.026.0555974
GGTATAC6800.025.7463571
GTATTAC6000.025.5318051
TCGTCCG1701.0824546E-425.4090335
TAGTACT7700.025.2452564
GTATAGA9450.024.7019731
GTATTAT9750.024.6900981
GTATAGT9000.023.505471
GTATAGG8150.023.2717591
CTCGCTC16400.022.8282833
GTGTAAA18550.022.8085421
TAACGGT1600.002266252922.4986674
GTCTCAC40450.022.3623091
Produced by FastQC (version 0.11.5)