FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345716

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345716
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2607626
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTGC23700.036.9606741
TCTGTCG12650.035.854558
GTACTAG5250.031.979781
GTCTTAC12050.031.5009211
GTCTCGC14300.028.0758951
TCGCTCT14550.027.7094334
GTGTTAG17800.027.4765221
GTATTAG8750.026.6961671
TCCGTCG1400.001041625225.7120238
GGGTTTC43600.025.2811851
CTGTCGC17700.025.2176349
GTTTCAC37700.025.0158863
GGAGTGC62400.022.9603985
GAGTGCA68400.022.7357126
AACCGTA1902.3011272E-422.7348427
TCTCGCT16850.022.6444572
TTGCTCT35300.022.6386684
GTATTAA8400.022.5944121
CGCTCTG15950.022.1180235
GTGTAAA17500.022.1077631
Produced by FastQC (version 0.11.5)