FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345717

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345717
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2988490
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13300.042.76438
GTCTCGC13150.042.688531
TCGCTCT15150.038.0187454
CTGTCGC16050.037.6799359
GTCTTGC27300.035.7839621
TCTCGCT15650.034.964472
CGCTCTG16150.033.4344865
CTCGCTC17100.031.157553
GTATTAG10600.027.510621
TTCACCG8450.026.4125845
GGTATAC6250.025.6619071
TTGCTCT39050.025.2592224
GTTTCAC45200.024.8492833
GGGTTTC49950.024.6659281
GTGTTAG21750.024.1334551
GTGTTAA12750.024.0151541
GTATTGG9150.023.902671
GTATAAT8550.023.8747121
GTGTTAC11250.023.3290061
GTACTAT5950.023.2799951
Produced by FastQC (version 0.11.5)