FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345718

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345718
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2988490
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC14350.040.7960621
TCGCTCT15300.036.7009354
TCTGTCG14950.036.1161428
GTCTTGC29100.032.4397051
TCTCGCT17350.031.5346572
CTCGCTC16950.031.428873
TAATACG2003.4655022E-728.7961224
CGCTCTG17800.027.906825
GTCTCAC37050.026.8615381
CTGTCGC20200.026.0167339
GTGTAAG13950.025.7040791
TTCACCG9550.024.8766465
GTATTAA8250.024.836151
GTATAAG8300.024.6865351
GTGTTAG21450.024.5632251
GTAATAC6900.023.9963653
GTTTCAC42600.022.9824353
GTACTAG6050.022.9815031
GAGTACG2552.4995825E-622.9577851
TTGCTCT42350.022.9485034
Produced by FastQC (version 0.11.5)