FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345719

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345719
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3193910
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12700.040.8137058
GTCTCGC15800.037.8728261
TCGCTCT17200.036.834834
CTGTCGC15500.034.3698239
GTATTAT9250.033.9232671
TCTCGCT19100.030.5325372
CGCTCTG19450.029.6110945
CTCGCTC19950.029.2316533
GTCTTGC29300.028.8909171
GTGTAGT18600.026.6788371
GTGTTAG22800.024.9649491
TTACACT15800.023.6946744
GTCCTAT9900.023.5877061
GTATTAA10600.023.4069041
GTATAAT8950.022.831
GTTTCAC48250.022.8299713
GTATTAC7950.022.030031
GTGTAAC11400.021.7643151
GGGTTTC56500.021.6986051
CTGTTGC32550.021.6746649
Produced by FastQC (version 0.11.5)