FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345720

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345720
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3193910
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG15000.038.3949178
TCGCTCT15300.032.9373364
GTCTCGC13350.032.3926241
GTCTTGC27150.030.2359491
GTATTAC7200.026.4677051
GTATTAA9900.025.9124371
CTCGCTC18500.025.6826863
GTATAGG8400.025.3042891
CTGTCGC22450.025.0118879
GTATAGT8800.024.9869921
TAGTACG1751.3180567E-424.6828354
CGCTCTG18600.024.3846345
TCTCGCT17950.024.0636672
ATAGTAC8000.023.3965383
GTCTTAC13900.023.2013511
GTGTTAC14600.023.0931
GTATAAG8650.022.8782621
GTGCTAC12650.022.5969311
TTGCTCT40700.022.2873254
GTATTAT9550.022.2571741
Produced by FastQC (version 0.11.5)