FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345721

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345721
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3093332
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11950.042.1695868
CTGTCGC15700.036.224029
GGTATAC7900.031.3851281
GTCTCGC13700.030.8730491
GTCTTGC27600.026.9502741
TCGCTCT14500.026.8124874
GTGTAGA17450.025.4921511
CTCGCTC17050.024.06963
TCTCGCT16000.023.8492242
GTATTAG10500.023.6135731
GTATAGG7550.023.1812251
GTACAAC7700.022.7296431
GTATATA14150.022.6760981
GTGTTAG21250.022.649421
GTATAAG9350.022.6182211
GTATTAA11300.022.5871641
GTGTAAC11400.022.389031
ACCGTGT11900.022.3832938
GTGTAAG17400.022.2125641
GTATAGT7900.022.154211
Produced by FastQC (version 0.11.5)