FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345724

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345724
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3195194
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTGC29000.031.8296131
TCTGTCG14050.030.7438188
GTCTCGC14100.029.615191
TCGCTCT15350.029.548044
TCTCGCT18000.025.998252
CTCGCTC18200.025.3161873
GTGTAAC9500.023.9054471
GTATTAG9000.023.6055581
TACACTG20000.023.3980625
TTACACT16350.023.3375154
GTCTCAC36900.023.0298141
CGCTCTG17600.022.4981335
GGTATAC6200.022.4502261
CTGTCGC20500.022.1236119
GTGTTAG19600.022.0523431
GAGTGCA77400.021.8576266
GTTACGC1650.002710312421.816033
GGAGTGC72350.021.792255
GTTAGCC17600.021.679683
GTCTAGC13650.021.467731
Produced by FastQC (version 0.11.5)