FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345725

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345725
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3431502
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG14500.042.698198
GTCTCGC15550.035.1697921
CTGTCGC19100.034.6763579
GTCTTGC32700.033.2259561
TCGCTCT17200.029.7202994
CGTTTCG2105.3093754E-727.4251929
TCTCGCT18550.027.1704242
CGCTCTG20300.026.5980955
CTCGCTC19250.025.4343413
GTATTAT11450.024.8369391
GTGTTAC13900.024.1313691
GTATTAA10200.023.5913451
GTGTTAT14750.023.2351111
GTATAGA11100.022.9923821
GTGTAGT21750.022.4623131
GTATTAG12800.021.647741
GGAGTGC79600.021.5253015
GTGTAAC12600.021.4126341
GTGTAAT14350.021.3420581
GTATTAC9250.021.2843761
Produced by FastQC (version 0.11.5)