FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345726

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345726
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3431502
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC17800.038.2524571
TCTGTCG15200.032.680468
TCGCTCT19900.032.5590864
GTCTTGC29250.029.285751
TCTCGCT22400.027.639692
CGCTCTG21250.026.7639945
GTATTAT11850.026.567241
GTGTTAG23600.026.0593551
CTCGCTC22950.025.094433
GTATAAC8450.024.2603841
GGTATAC7900.023.1691061
CTGTCGC23450.022.7177479
GTATTAA11150.022.325461
GTATATA15600.022.0581761
GTGTAAA23750.021.2707021
ACCGTGT13050.020.9631168
GTGTTAC13750.020.7662581
GGGTTTC57400.020.2806361
GTCTTAC16250.020.2747521
GTATAGG9800.020.1713071
Produced by FastQC (version 0.11.5)