FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345727

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345727
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2730266
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10900.043.5896768
CTGTCGC13500.036.7977529
GTATTAT8150.034.016451
GTCTTGC27350.032.543591
TCGCTCT13250.030.4272164
GTCTTAC10850.028.9135681
TCCGTCG1255.374054E-428.7956058
GTATTAC6550.027.8459281
GTCTCGC12850.027.8199231
GTCTCAC33500.026.5691721
GTATTGC7350.025.807681
GTATAGA8250.025.6452541
GTGTTAG19700.024.812561
GTACTAT6500.024.6929111
GTTAGCC15200.024.6291733
GTACTAC5050.024.5595571
GTATTAA6450.023.7532251
TTACACT12800.023.6226924
TTCACCG8350.023.2778845
GTTTCAC40900.023.2348943
Produced by FastQC (version 0.11.5)