FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345728

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345728
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2730266
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11700.040.5867778
GTCTCGC11600.034.054941
CTGTCGC15400.030.3682089
TCGCTCT13000.028.7797164
GTCTTGC25300.027.180141
GGGTTTC42600.025.5870951
GTATTAA7750.025.4862751
TCTCGCT16450.024.9339542
CTCGCTC15500.024.6042693
GTGTAAA16450.024.4591371
GTATTAG8100.024.3850151
CGCTCTG14150.023.3898745
GTCTAAC6600.023.2766081
GAGTGCA68700.023.249996
GTGTTAC9550.022.9806441
GGCTAGA17900.022.8864961
GTGTTAG17150.022.6076851
GTTTCAC41700.022.6048453
TTGCTCT33300.022.470654
GTATTAC5900.022.3184911
Produced by FastQC (version 0.11.5)