FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345729

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345729
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2443728
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9500.051.5305448
TCGCTCT11350.038.69214
CTGTCGC12400.037.7388659
GTCTCGC12400.037.6505471
GTCTTGC24050.035.1848681
GTATAAG6850.031.9480041
TCTCGCT14650.031.4507242
CTCGCTC14500.030.7830773
TAGTTCG1652.523113E-630.5417237
GTATTAT8600.030.5363451
GTGTTAG17650.028.9311941
TTGCTCT33550.028.1104034
GTATAAC4250.027.4627531
GTATTAA7350.026.7972011
TAATACT8100.026.6639544
CGCTCTG13850.026.5093965
GTTTCAC35250.025.5293183
GTATAAT6900.025.3731961
GTGTTAT10000.024.80231
CTTGCTC33400.024.7878973
Produced by FastQC (version 0.11.5)