FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345731

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345731
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2590714
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11200.046.922938
GTCTCGC11300.040.068841
CTGTCGC14400.037.9961629
TCGCTCT13000.033.7812044
GTCTTGC25550.032.584231
TCTCGCT13300.031.395332
CGCTCTG15650.028.0605245
CTCGCTC15100.027.652843
GTCTCAC31200.025.7472931
TAATACT8950.025.740424
GTACTAG5750.025.4012831
CGGTACT2001.1259917E-525.1974584
GTATTAG9350.024.9937761
GGGTTTC45150.024.909011
GTATTAT9100.024.8779051
GTTTCAC38150.024.5322573
GTATTAC6000.024.3428971
GTACACG3001.5827027E-824.3428971
GTGTTAC11450.024.2365971
GTACTAA7600.024.0225961
Produced by FastQC (version 0.11.5)