FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345733

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345733
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2654075
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11600.043.4443978
GTCTCGC12350.041.3348431
GTCTTGC25100.038.6423151
TCGCTCT15100.034.8054964
CTGTCGC14450.034.37959
TCTCGCT16450.031.0738012
GTGTTAG18100.030.6210581
CTCGCTC17100.029.4716073
GTCTTAC10950.027.9717961
CGCTCTG17700.027.6575075
GTGTAAA15800.027.2320371
TTGCTCT35800.026.9478234
GTATTAG8250.026.5187171
GTATAAC4550.025.6444721
GTTTCAC39950.025.4099773
GGGTTTC44600.025.0173761
CTTGCTC36500.024.8530453
GGTATAC5300.024.767481
TAACACT13150.024.6370474
GTGTAGG15400.024.1509741
Produced by FastQC (version 0.11.5)