FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345734

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345734
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2654075
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12000.045.5689858
GTCTTGC25750.035.4899861
GTCTCGC13000.033.180411
TCGCTCT13550.032.922864
CTGTCGC17200.031.3739959
CGCTCTG14850.028.1026135
CTCGCTC16350.026.8451253
GTATTAC7000.026.110491
TTGCTCT33800.025.75814
GTACTAT4900.025.3644771
GTATTAT9250.025.2918911
GTATAGG6700.025.0972461
TCTCGCT17650.024.8673972
GTGTTAG19150.024.8151931
CTTGCTC33800.024.6941833
CTTAGCG1801.6016919E-423.9836779
GTATAAG7450.023.5520131
GTTTCAC38500.023.5484963
TCTATCG2202.3655924E-522.8935078
GTGTTAC10450.022.387561
Produced by FastQC (version 0.11.5)