FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345735

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345735
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2654244
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10450.039.956118
GTCTCGC10900.038.862211
TCGCTCT13400.033.311264
CTGTCGC13150.032.8477139
GTCTTGC26150.032.676851
TCTCGCT14250.031.8295062
CTCGCTC14500.031.7772433
CGCTCTG13100.030.7737225
GTCGTAC1050.007439356327.8225351
GTATAAC5650.025.8527981
TTGCTCT36200.025.2580244
GTACTAG5400.024.3447171
GTGTTAG18200.024.0771921
GTATAAT8800.024.0680731
GTGTTAT11150.023.5805341
TTATACT10200.023.2925994
GTATTGA9550.022.9426651
GGGTTTC50950.022.7918151
GTATTAG9300.022.774091
CTTGCTC34650.022.6478753
Produced by FastQC (version 0.11.5)