FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345736

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345736
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2654244
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11500.042.5444078
GTCTCGC12900.036.896191
TCGCTCT13350.032.8766984
CTGTCGC15950.029.7724579
CGCTCTG14400.029.4801125
CTCGCTC14700.028.3922213
TCTCGCT16050.027.3486142
GTCTTGC23350.026.969291
GTATTAG7650.025.8440281
GTCTTAC11700.025.0341051
GTGTAAT11250.024.7336941
GTGTTAG17950.023.2524281
GTATTAT9750.022.5306951
CCGTGTT9950.022.4166169
CTATACT8200.021.936424
TTGCTCT34800.021.916254
TATACCG1650.002719266821.8034735
ACCGTGT10500.021.2424138
GTATAGG7950.021.184521
GGGTTTC46350.021.0116351
Produced by FastQC (version 0.11.5)