FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345737

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345737
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2439345
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9700.046.7565048
CTGTCGC11950.040.3668259
GTCTCGC10750.034.6218571
GTCTTGC23750.033.4928051
TCGCTCT11750.030.6372534
GTATTAT9200.028.556391
GTATTAC6250.028.023341
CTCGCTC13100.026.3807833
CGCTCTG13450.026.2269025
GTATAGC5100.025.7567441
TCTCGCT13800.025.5643482
GTATAAG7700.024.6417351
GTACCGG1801.4549002E-424.3258151
GTGTTAG17200.024.1843851
GTGTAGA12950.023.668361
GTGTTAT10850.023.5411111
GTATATA11550.023.3780561
ATAAGCG1851.921446E-423.3481645
GTATTAG9100.023.2565481
GTCTTAC9950.022.7366921
Produced by FastQC (version 0.11.5)