FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345739

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345739
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2477416
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10600.039.39148
CTGTCGC13200.034.905529
GTCTCGC10400.034.4212041
TCGCTCT11050.031.9243344
GTCTTGC23600.030.0277651
CTCGCTC12650.027.8864753
GTATTAG9250.027.6432841
TCTCGCT13100.026.9285432
GTATAAT7450.024.5158521
TCGATCT9800.024.2424169
CTCGATC9650.023.8727258
TTGCTCT33800.023.8555474
GTATAAC4301.8189894E-1223.786081
CGCTCTG13400.023.1011815
GTATAAG7300.023.0180381
GTGTTAC10500.022.2650661
GTGTAAC7550.022.255851
GTGTTAT10200.022.2036741
TCTCGAT10400.022.1511337
GTATATA9900.022.138561
Produced by FastQC (version 0.11.5)