FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345742

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345742
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2649392
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12650.046.071738
CTGTCGC17850.035.0688139
GTCTCGC12600.034.845311
TCGCTCT14550.032.637814
GTCTTGC23650.031.5596851
CGCTCTG15100.030.0195145
CTCGCTC15100.027.161543
TCTCGCT16350.025.526022
GTCTTAC11250.025.3673861
GTACTAT5600.024.8272821
GGGTTTC47450.023.7491511
GTATTAT9250.023.732481
GTCTCAC31250.023.6502081
GTGTTAG17300.023.2637731
GTATAAT9600.022.8672331
GTATTAC7050.022.8347991
TTACTCT17600.022.4848564
GTGTAAC8250.022.1742861
GTTTCAC41900.022.1529773
TTGCTCT35600.021.6258834
Produced by FastQC (version 0.11.5)