FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345743

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345743
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2516079
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8700.041.3741958
GTCTCGC11350.034.0658261
GTATTAT7550.032.8526881
GTCTTGC24950.031.2861541
CTGTCGC12500.031.1001559
GTATTAA8850.029.675511
TCGCTCT13100.028.5805934
GTATTAG9350.026.5281071
CGCTCTG13950.026.3188025
TCTCGCT14200.025.8595542
TTGCTCT34150.025.0896994
GGTATAC5350.024.5446971
GTCTCAC30250.023.8752961
GTATAGG7650.023.840621
GTTTCAC40800.023.8238963
CTCGCTC14550.023.7529453
GTGTAAA17200.023.751911
GGGTTTC45900.023.6816831
TAGCACG3352.2901077E-923.6421574
GTATAAA10400.023.1483231
Produced by FastQC (version 0.11.5)