FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345744

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345744
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2516079
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13050.036.940148
GTATAAC4950.035.468741
GTCTCGC11850.035.1881331
GTCTTGC26950.033.9305531
GTATTAG9550.033.7045861
TCGCTCT13700.030.9866264
CTGTCGC16950.030.1385889
GTGTTAC10700.028.7147621
TCTCGCT16150.027.6246362
CTCGCTC15650.026.2077223
TTGCTCT35150.025.3827824
CGCTCTG16200.025.3164445
GTCTCAC31900.025.2256131
GTATAAT8750.024.2454171
GTGTTAG16600.024.2378641
GTCTTAC10600.024.1547131
CGTTTCG2151.9803825E-523.4257649
GTGTAAG12450.022.9157981
CTTGCTC35300.022.4226383
GTATTGG8500.022.3766021
Produced by FastQC (version 0.11.5)