FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345746

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345746
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2403121
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10650.036.4835558
GTCTTGC23100.031.074771
GTCTCGC10450.029.4392591
GTATTAG9000.029.299071
GTACCGT1856.174074E-627.225156
TTCACCG6100.027.1295095
GTATAAT6500.027.0452941
TCGCTCT12100.026.7585184
GTCTTAC9450.026.353661
ATACCGT1658.869307E-526.1644276
CTGTCGC15300.025.8656989
GTATAGG6700.025.1447241
GTATTAA6550.024.6022741
CTCGCTC12800.024.1719343
TATACGG1801.6014578E-423.9840582
GTGTTAC11300.023.9837511
GTGTAAA15050.023.848081
GTATATA11850.023.4887051
ACCGTGT8000.023.3849438
GTGTTAG15350.022.904811
Produced by FastQC (version 0.11.5)